细胞适应性猪流行性腹泻病毒部分基因序列分析及其致病力研究

doi:10.11843/j.issn.0366-6964.2015.05.015

Abstract

Abstract:

In this study，we further maintained the porcine epidemic diarrhea virus CHYJ130330 in Vero cells up to the 100th passage and analyzed changes in the spike（S），membrane（M），and nucleocapsid（N） gene sequences and pathogenicity of the virus at the 38th，60th，80th，and 100th passage levels.Sequence analyses revealed a strong selection for the S gene of CHYJ130330 in Vero cells，and all mutations occurring at the 60th，80th，and 100th-passage virus.In contrast，the viral M and N genes showed a strong conservation during the serial passage.Pigs that experimentally infected with the 60th and 80th-passage virus，but not the 100th-passaged virus，exhibited vomiting and diarrhea，indicating an attenuation of the CHYJ130330 at the 100th passage.Further studies will be required to define whether the mutations in the S gene of CHYJ130330 that had been selected and accumulated during the serial passages are indeed the causalities of the growth adaptation in vitro and the attenuation of virulence in vivo.

CLC Number:

S852.659.6

FENG Xiao-sheng，LIU Qi，WANG Yue-yun，ZHOU Ru-yue，AN Xin-yu，WANG Gui-ping，JIA Ai-qing. Mutations in Partial Genes of Porcine Epidemic Diarrhea Virus Associated with Growth Adaptation in vitro and Attenuation of Virulence in vivo[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 792-799.

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Mutations in Partial Genes of Porcine Epidemic Diarrhea Virus Associated with Growth Adaptation in vitro and Attenuation of Virulence in vivo

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